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biotinylated goat anti-mouse igg antibody  (Vector Laboratories)


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    Vector Laboratories biotinylated goat anti-mouse igg antibody
    Biotinylated Goat Anti Mouse Igg Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 7439 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat anti-mouse igg antibody/product/Vector Laboratories
    Average 96 stars, based on 7439 article reviews
    biotinylated goat anti-mouse igg antibody - by Bioz Stars, 2026-04
    96/100 stars

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    Pharmacological inhibition of SIK2/SIK3 increases bone formation. (A) Representative H&E from tibia histologic sections from all treatment groups. Scale bar: 100 μm. (B) Representative fluorochrome-labeled femur histologic cross sections. Primary spongiosa (upper panel) and metaphyseal cortical (lower panel). Highly magnified double-calcein labeling images from all treatment groups. Scale bar: 100 μm. (C) Quantification of bone dynamic histomorphometry histological section derived measurements: MS/BS, MAR, and BFR were measured using the double calcein labels, which were injected at day 9 and day 2 before sacrifice timepoints. (D) Representative tibia sclerostin immunohistochemistry (DAB staining) sections from sham vehicle and all ORX treatment groups. Scale bar: 20 μm. (E) Quantification of Sost positive cells from sclerostin immunohistochemistry staining. Positive cells were counted at midshaft cortical region. (F) Relative gene expression levels of Sost to β- actin (left) and Tnfsf11 (right) to β- actin from marrow flushed cortical bone RNA from sham vehicle and all ORX treatment groups. All data were analyzed using one-way ANOVA followed by Dunnett’s post-hoc tests within sham or ORX groups, vs vehicle ( * p < .05; ** p < .01; *** p < .001). All values are indicated as mean ± SD. For MS/BS and endo-cortical MAR of panel C, n = 8 per group for vehicle and SK-124. n = 7 per group for PTH. For MAR and BFR of panel C, n = 8 per group for vehicle and SK-124. n = 7 per group for PTH in sham groups. n = 8 per group for ORX vehicle, n = 7 per group for ORX SK-124, and n = 4 per group for ORX PTH. n = 3 per group for panel E. For panel F, n = 5 per group for ORX PTH. n = 6 per group for ORX vehicle. N = 7 per group for sham vehicle and ORX SK-124.

    Journal: JBMR Plus

    Article Title: The orally available SIK2/SIK3 inhibitor SK-124 increases bone mass in hypogonadal male mice

    doi: 10.1093/jbmrpl/ziag032

    Figure Lengend Snippet: Pharmacological inhibition of SIK2/SIK3 increases bone formation. (A) Representative H&E from tibia histologic sections from all treatment groups. Scale bar: 100 μm. (B) Representative fluorochrome-labeled femur histologic cross sections. Primary spongiosa (upper panel) and metaphyseal cortical (lower panel). Highly magnified double-calcein labeling images from all treatment groups. Scale bar: 100 μm. (C) Quantification of bone dynamic histomorphometry histological section derived measurements: MS/BS, MAR, and BFR were measured using the double calcein labels, which were injected at day 9 and day 2 before sacrifice timepoints. (D) Representative tibia sclerostin immunohistochemistry (DAB staining) sections from sham vehicle and all ORX treatment groups. Scale bar: 20 μm. (E) Quantification of Sost positive cells from sclerostin immunohistochemistry staining. Positive cells were counted at midshaft cortical region. (F) Relative gene expression levels of Sost to β- actin (left) and Tnfsf11 (right) to β- actin from marrow flushed cortical bone RNA from sham vehicle and all ORX treatment groups. All data were analyzed using one-way ANOVA followed by Dunnett’s post-hoc tests within sham or ORX groups, vs vehicle ( * p < .05; ** p < .01; *** p < .001). All values are indicated as mean ± SD. For MS/BS and endo-cortical MAR of panel C, n = 8 per group for vehicle and SK-124. n = 7 per group for PTH. For MAR and BFR of panel C, n = 8 per group for vehicle and SK-124. n = 7 per group for PTH in sham groups. n = 8 per group for ORX vehicle, n = 7 per group for ORX SK-124, and n = 4 per group for ORX PTH. n = 3 per group for panel E. For panel F, n = 5 per group for ORX PTH. n = 6 per group for ORX vehicle. N = 7 per group for sham vehicle and ORX SK-124.

    Article Snippet: Next, slides were blocked in TNB buffer (Perkin-Elmer), stained with anti-sclerostin biotinylated antibody (1:50, R&D Systems, BAF1589) overnight at 4 °C.

    Techniques: Inhibition, Labeling, Derivative Assay, Injection, Immunohistochemistry, Staining, Gene Expression